What is the meaning of recombinant protein?
Recombinant protein expression
Transfecting a cell with a DNA vector containing the recombinant DNA template is one method of producing recombinant protein. The cells that carry the template are then grown in order for them to transcribe and translate the desired protein.
After that, the cells can be lyzed or broken to release the produced protein, which can then be purified using various procedures. Both prokaryotic and eukaryotic systems are employed in the expression of recombinant DNA. This decision is frequently made based on the protein type, functional activity, and necessary yield.
Mammalian ecologies
Functional tests, structural analysis, antibody synthesis, protein interaction, virus production, and other processes are all carried out in mammalian systems. The ability to produce proteins in either a transitory or stable manner is a major benefit of this approach.
Furthermore, the system has been designed to produce proteins quickly and with a high yield. The need for big yields (such as gram/liter) in suspension cultures, on the other hand, is one of the downsides. In this situation, the cell culture conditions are also more demanding.
