Retrogen, Inc. | How Does The Sanger Sequencing Works?
This procedure again helps in the separation of the extension productsby their size specifically at one base resolution. As the laser passes througha little window present at the capillary end, the DNA with plasmid preparationfragments which are labeled by the dye are excited. In this case, alight sensor detects light at a specific wavelength which is emitted by theexcited dye. Then, the software can detect the signal and thereby translate itinto a base cell.
Whenever the NGS Sequencing reaction takes place particularly in thepresence of 4 terminated nucleotides, you will get a pool of the DNA fragmentswhich are measured and also separated base by base. So, this is how thisprocess takes place. One important thing that you should know about is thatboth Sanger Sequencing and PCR make use of similar starting materials. View More
