In-situ hybridization is a laboratory technique that allows researchers to pinpoint the location of specific DNA/RNA sequences within chromosomes. The ability of the DNA helix to re-form or re-nature when given favorable conditions underpins molecular hybridization. A labeled DNA or RNA sequence is used as a probe in molecular hybridization to identify/locate or quantify the naturally occurring DNA or RNA sequence in a biological sample. A nucleic acid probe is created, labeled, purified, and annealed with a specific target. It is a critical technique in genetic research and the discovery of gene-based medicine, particularly in personalized medicine.
The hybridization technique is frequently used to locate specific nucleic acid sequences on chromosomes or in tissues in order to better understand gene organization, regulation, and function. The in-situ hybridization technique broadens the capabilities to meet any therapy needs and enable new diagnostic and treatment applications for cancer and other chronic diseases patients.
Its unique technology for detecting aneuploidies during the prenatal stage ensures a better functional ability for detecting abnormalities associated with birth defects or mental disorders. In situ hybridization is particularly beneficial in neuroscience. Microbiology, pathology, developmental biology, karyotyping and phylogenetic analysis, and physical mapping all benefit from in situ hybridization.
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