A simple and sensitive spectrophotometric method for determining roxithromycin in pharmaceutical dosage forms has been developed. The proposed method involves oxidizing roxithromycin with potassium permanganate to liberate formaldehyde, which is then determined in situ with acetylacetone in the presence of ammonium acetate to yield a yellow-colored chromogen with absorption maxima at 412 nm. In the concentration range of 10-75 g/ml, the method is found to be linear, with a regression coefficient of 0.9987. When two-tailed t-tests were used, there was no significant difference between the proposed method and the reported method.
Several reaction parameters, including potassium permanganate and reagent concentrations, oxidation time, and maximum color intensity, were optimized. The method has been validated and can successfully be used to assay roxithromycin in its pharmaceutical dosage form, namely tablets.
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