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The MAT provides an in vitro alternative to traditional animal testing in conformity with regulatory requirements. It is used to identify endotoxins and non-endotoxin pyrogens in parenteral items, such as medications and medical equipment.
Pyrogen testing commonly involves the Limulus Amebocyte Lysate (LAL) test and the Rabbit Pyrogen Test. Both techniques have drawbacks and involve the use of animals. Since an animal's reaction might be very different from a person's reaction, the rabbit pyrogen test demonstrates a lack of resilience. By ignoring pyrogens other than endotoxins that might be present in the sample being analysed, the LAL test only detects endotoxins, posing a safety risk.
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