A significant tool utilised in the domains of cell biology and biomedicine is single-cell analysis (SCA). Modern, superior optical technology served as the foundation for the conception and growth of Single Cell Analysis. Optically tweezing, super-resolution optical microscopy, dynamic light scattering, fluorescence imaging, evanescent-wave sensing, and hyperspectral microscopy have all been employed to identify SCA thus far. Surface plasmon resonance (SPR), plasmon waveguide resonance (PWR), and metal-free waveguide resonance (WGR) sensors are the most common optical evanescent-wave sensors used for SCA. All of these sensors are label-free and noninvasive. The aforementioned sensors are extremely sensitive to changes in refractive index brought on by chemical and biological interactions on the surface because optical evanescent wave is a nonradiated surface electromagnetic wave.
However, Whole Genome Sequencing or Whole Exome-Sequencing (WGS or WESseq) in human single-cell genomics has a lot of potential for clinical applications, particularly in screening, diagnosis, and monitoring. Single Cell Analysis explains life at its most basic level and has numerous medical applications. Because mRNA shows gene functions and regulatory networks, bridges genome structure and epigenomics modification with phenotype, and is easier to accomplish than proteomics, it is well-liked in academic research. New generation techniques, including third generation sequencing and next generation sequencing, are very important in the field of genomics. Measurement and study of cellular heterogeneity are the main goals of single cell analysis.
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