Global Immobilized Trypsin MarketDrivers
Leading manufacturers areinvolved in manufacture of immobilized trypsin enzyme with the reduced time ofincubation in the global ImmobilizedTrypsin Market. For example, the immobilized trypsin manufactured by GenoTechnology Inc. and Promega Corporation requires a sample to be incubated inthe spin columns for 2-18 hrs. And for 30 mins at 37 degrees celsius,respectively. In case of, Merck KGaA manufactured product, trypsin spin columnswith immobilized trypsin, the sample has to be incubated in the columns for 15mins at room temperature. Therefore, the incubation time of the sample with theimmobilized trypsin has been decreased in case of Merck KGaA manufacturedproduct. This can benefit the researchers by rapid protein digestion forprotein analysis.
Immobilized trypsin finds manyapplications in fundamental research. For instance, it is used in affinitychromatography for the purification of trypsin inhibitors from the solution.Immobilized trypsin finds many applications in research for studying protein –protein interactions as well as protein identification in systems biology toidentify drug targets in the cells. Therefore, use of immobilized trypsinoffers an advantage in scientific research as the technique is cost-effective.Apart from these applications, immobilized trypsin is used in other fields suchas to identify the post translational modifications. Other advantages of usingimmobilized trypsin are, easy to use, wide spread availability, and highspecificity. The above factors are expected to boost the global immobilizedtrypsin market growth over the forecast period.
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Trypsin is a serine proteaseenzyme (EC number of 3.4.21.4), which hydrolyses esters of basic amino acidssuch as lysine and arginine in the peptides. Optimal temperature for trypsinactivation is around 37 degrees Celsius and its optimal pH is around 7.5 to9.0. Trypsin is available in the form of immobilized enzyme where it isattached to an inert support such as dextran, sephadex, cellulose, starch etc.Immobilized Trypsin is supplied in preservatives such as glycerol and sodiumazide as a slurry. It finds applicationsin research for the purpose of protein identification, proteincharacterization, study of post translational modifications, and protein-proteininteractions.
There are many manufacturersproducing immobilized trypsin like Thermo Fischer Scientific who manufacturesimmobilized trypsin, treated with L-1-tosylamido-2-phenylethyl chloromethylketone (TPCK) to inhibit chymotrypsin activity without altering the activity oftrypsin, in order to prevent contamination. Some of the other manufacturers arePromega Corporation, Merck KGaA, etc.
Global Immobilized Trypsin MarketRestraints
According to the National Centerfor Biotechnology Information (NCBI), 2017, disadvantages of using trypsin areautolysis, intolerance to high temperatures or organic solvents and missing outon particular sites of post-translational modifications, protein segments oreven subsets of proteins. To overcome this drawback, other enzymes are beingtested for the catalytic function, which would act as an alternatives forimmobilized trypsin. For instance, according to National Centre forBiotechnology Information, (NCBI), 2016, the catalytic function of the sixproteases (Chemotrypsin, LysC, LysN, AspN, GluC and ArgC,) was researched andthe proteases have been shown to offer promising results. The above factors areexpected to restrain the global immobilized trypsin market growth over theforecast period.
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Global Immobilized Trypsin MarketRegional Analysis
North America is expected towitness significant growth in the global immobilized trypsin market over theforecast period, owing to the manufacture of modified immobilized trypsinenzyme with increased throughput and confidence in results. For instance, in2015, Thermo Fisher Scientific launched, Thermo Scientific Digest Kit, aprotein analysis kit useful for applications related to biopharmaceuticals. Thekit includes a thermostable immobilized trypsin for reducing digestion time andfor reproducing high-quality data.
Moreover, headquarters of keymanufacturers have wide-spread presence in the North America region. Forinstance, Promega Corporation, Merck KGaA, and Thermo Fisher Scientific arelocated in the U.S. Therefore, the above factors are expected to boost theglobal immobilized enzymes market growth over the forecast period.
Global Immobilized Trypsin MarketKey Players
Some of the key players operatingin the global immobilized trypsin market are Promega Corporation, Merck KGaA,Geno Technology Inc., and Thermo Fisher Scientific
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