How To Get The Ideal Outcome With Your ELISA Kits?
An ELISA or EIA referred to as enzyme-linked immunosorbent assay is one of the most proficient tests to measure and detect blood antibodies. Laboratories utilize this test to determine if a being has specific antibodies related to severe infectious conditions. The next step is to select the right ELISA type.
There are basically four types of ELISA -
1. Direct ELISA
2. Indirect ELISA
3. Sandwich ELISA
4. Competitive ELISA
Most important points to be noted while Getting Suitable Results with ELISA
1. Choose the surface wisely.
2. Chequerboard
3. Proper sample dilution is vital.
4.No equal antibodies
5. Understand blocking surprises
6. Secondary antibodies - Batch testing
7. Consider normalizing
8. Know your dynamic range
9. Follow the recommended temperatures, incubation times, and conditions.
10. Obtain data analysis and optimal detection.
